10+ puasp-attb vector

In order to over-express constructs in S2 cells cDNAs corresponding to GFP sh3px1 SNX9 SNX18 and SXN33 were cloned into the pUASp-attB-K10 vector Koch et al 2009. Expression of the tagged proteins was induced by co-transfection with a plasmid expressing Gal4 driven by the Actin5C promoter gift of Jocelyn McDonald Kansas State University.


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